Fluorescein Isothiocyante CAS: 3326-32-7

Imiterere y'urumuri: FITC ifite umusaruro mwinshi wa quantum kandi itanga urumuri rw'icyatsi kibisi iyo ishyushye ku gipimo cya 488 nm. Imiterere yayo y'urumuri ituma ikoreshwa mu gupima hakoreshejwe uburyo butandukanye bwo gufata amashusho, nka mikorosikopi ya fluorescence, flow cytometry, na fluorescence spectroscopy.

Guhuzanya kwa Covalent: FITC irimo itsinda rya isothiocyanate riyifasha guhuzanya n'amatsinda ya amino ari muri poroteyine, antibody, n'izindi biomolecules. Uku guhuzanya kwa covalent gutuma molekile zigomba gushyirwaho ikimenyetso gihamye kandi cyihariye, bigatuma zimenyekana kandi zigashobora kugaragara.

Ubudahangarwa bw'umubiri: Ubudahangarwa bw'umubiri bufite ikimenyetso cya FITC bukunze gukoreshwa mu isuzuma ry'ubudahangarwa bw'umubiri kugira ngo hamenyekane antigen cyangwa poroteyine runaka mu turemangingo no mu turemangingo. Ubudahangarwa bw'icyatsi kibisi butangwa na FITC butuma abashakashatsi bashobora kubona no kwiyumvisha uko molekile zigomba gukwirakwira mu bipimo by'ibinyabuzima.

Cytometry y'imitsi: Antibodies zanditseho FITC cyangwa izindi biomolecules za fluorochrome zikoreshwa cyane muri cytometry y'imitsi kugira ngo zisesengure kandi zitondeke uturemangingo hashingiwe ku bimenyetso byazo cyangwa ibice byazo biri mu ngirabuzima fatizo. FITC irahuye na laser ya 488 nm ikoreshwa cyane mu bikoresho bya cytometry y'imitsi.

Inyigo ku mikoranire ya poroteyine: FITC ishobora gukoreshwa mu nyigo ku mikoranire ya poroteyine na poroteyine kugira ngo hamenyekane uburyo poroteyine zifatana n'uko zikorana. Mu gushyira poroteyine runaka mu izina rya FITC, abashakashatsi bashobora gusesengura imikoranire yazo bakoresheje uburyo nka fluorescence resonance energy transfer (FRET) cyangwa fluorescence polarization.

Gukurikirana ingirabuzimafatizo z’ibinyabuzima: FITC ishobora gukoreshwa mu gukurikirana no gukurikirana urujya n’uruza rw’ingirabuzimafatizo z’ibinyabuzima cyangwa ingirabuzimafatizo. Mu gushyira ibirango bya FITC ku binyabuzima, abashakashatsi bashobora kwiga aho biherereye, imiterere yabyo, n’uburyo bicuruzwa mu buryo bwihuse hakoreshejwe mikorosikopi y’urumuri.

Uburyo bwo gusuzuma indwara: Ibikoresho cyangwa antibodies byanditseho FITC bikoreshwa mu gusuzuma indwara mu buryo butandukanye, harimo no kumenya antigens runaka, ibintu byandura, cyangwa ibimenyetso byerekana ibimenyetso mu barwayi. Ibi bikoresho bitanga uburyo bwo gusuzuma indwara mu buryo bwihuse kandi bufatika, bigatuma umuntu amenya indwara vuba kandi neza.