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N, N-Bis (2-hydroxyethyl) -2-aminoethanesulfonic acide sodium umunyu CAS: 66992-27-6
N.Ifasha kugumana urwego ruhamye rwa pH, bigatuma biba byiza mubikorwa nkumuco wimikorere, insimburangingo ya enzyme, ubushakashatsi bwa poroteyine, electrophorei, hamwe nubuvuzi bwa farumasi.HEPES umunyu wa sodiumi itanga uburyo bwiza bwibinyabuzima kandi bikongerera ukuri no kwizerwa ibisubizo byubushakashatsi.
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S-Butyrylthiocholine iyode CAS: 1866-16-6
S-Butyrylthiocholine iyode ni imiti ivanze ikunze gukoreshwa mubushakashatsi bwibinyabuzima na enzymatique.Ni substrate ya enzyme butyrylcholinesterase (BChE) kandi ikoreshwa mugupima ibikorwa byayo.
Iyo S-Butyrylthiocholine iyode ihinduwe na BChE, itanga thiocholine na aside butyric nkibicuruzwa.Isohora rya thiocholine rirashobora gupimwa ukoresheje spropropotometometric cyangwa fluorometric assay, bigatuma umubare wibikorwa bya BChE ugabanuka.
Iyode ya S-Butyrylthiocholine ikoreshwa kenshi mubuvuzi nubushakashatsi kugirango isuzume ibikorwa bya BChE mubitegererezo nka plasma yamaraso cyangwa tissue.Irashobora gukoreshwa mugusuzuma imikorere yimikorere ya BChE ninshingano zayo mubikorwa bitandukanye byibinyabuzima, ndetse no gusuzuma no gukurikirana indwara zimwe na zimwe.
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ABTS (2,2′-Azino-bis (3-Ethylbenzthiazoline-6-sulfonique aside) umunyu wa diammonium) CAS: 30931-67-0
Diammonium 2,2′-azino-bis (3-Ethylbenzothiazoline-6-sulfonate), bakunze kwita ABTS, ni insimburangingo ya chromogeneque ikoreshwa mubushakashatsi bwibinyabuzima, cyane cyane mubijyanye na enzymologiya.Nibintu bya sintetike ikoreshwa mugupima ibikorwa byimisemburo itandukanye, harimo peroxidase na okiside.
ABTS idafite ibara muburyo bwa okiside ariko ihinduka ubururu-icyatsi iyo ihinduwe na enzyme imbere ya hydrogen peroxide cyangwa ogisijeni ya molekile.Ihinduka ryamabara riterwa no gushiraho radical cation, ikurura urumuri muburyo bugaragara.
Imyitwarire hagati ya ABTS na enzyme itanga ibicuruzwa byamabara ashobora gupimwa spekitifoto.Ubwinshi bwamabara buragereranywa nigikorwa cyimisemburo, bituma abashakashatsi basuzuma mu buryo bwuzuye enzyme kinetics, kubuza enzyme, cyangwa imikoranire ya enzyme-substrate.
ABTS ifite uburyo bwinshi bwo gukoresha mubice bitandukanye, harimo kwisuzumisha kwa muganga, ubushakashatsi mu bya farumasi, na siyanse y'ibiribwa.Irumva cyane kandi itanga intera nini yingirakamaro, bigatuma ihitamo gukundwa kubinyabuzima byinshi.
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4-NITROPHENYLΑ-D-MALTOHEXAOSIDE URUBANZA: 74173-30-1
4-Nitrophenyl α-D-maltohexaoside ni uruganda ruri mu cyiciro cya α-glycosidic.Nibikomoka kuri maltose, ikaba disaccharide igizwe nibice bibiri bya glucose.Muri uru ruganda, hydroxyl groupe ya glucose yambere isimburwa na nitrophenyl moiety.
Uru ruganda rusanzwe rukoreshwa nka substrate mubisubizo byimisemburo yo kwiga ibikorwa byimisemburo itandukanye, cyane cyane abagira uruhare muri metabolism ya karubone.Itsinda rya nitrophenyl ryemerera gutahura no kugereranya ibisubizo byimisemburo mugupima kwinjiza cyangwa fluorescence yibicuruzwa byacitse.
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PIPES CAS: 5625-37-6 Igiciro cyabakora
PIPES (piperazine-1,4-bisethanesulfonic aside) ni uruganda rwa zwitterionic rukoreshwa cyane mubushakashatsi bwibinyabuzima na biohimiki.Nibikorwa byiza bya pH bifite ubushobozi buke bwo kubungabunga imiterere ihamye ya pH murwego rwa pH ya 6.1 kugeza 7.5.PIPES ifite intera ntoya na biomolecules kandi irakwiriye kubushakashatsi bushingiye ku bushyuhe.Bikunze gukoreshwa mubuhanga bwa gel electrophorei no gukora imiti nkumuti uhoraho.Muri rusange, PIPES ni ibintu byinshi kandi bikoreshwa cyane mubice bitandukanye byubushakashatsi.
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3,3 ′, 5,5′-Tetramethylbenzidine CAS: 207738-08-7
3,3 ′, 5,5′-Tetramethylbenzidine, izwi kandi ku izina rya TMB, ni imiti y’imiti ikunze gukoreshwa nka chromogenic substrate mu misemburo ifitanye isano na immunosorbent ya enzyme (ELISA) hamwe nubundi bushakashatsi bwibinyabuzima.Bikunze gukoreshwa mugushakisha no kugereranya ahari enzymes nka horseradish peroxidase (HRP) mubitegererezo bitandukanye byibinyabuzima.TMB ihinduka ibara kuva ibara ridafite ibara ry'ubururu imbere yiyi misemburo.Ibikurikira, reaction irashobora guhagarikwa wongeyeho aside ihindura ibara ryubururu kumabara yanyuma yumuhondo.Ubwinshi bwamabara yumuhondo buringaniye nubunini bwa enzyme ihari, yemerera kubara.
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APS-5 CAS: 193884-53-6 Igiciro cyabakora
.Igizwe na sisitemu ya 10 ya methylacridine hamwe na tsinda ryibice bitatu bifatanye na 4-chlorophenyl.Uru ruganda kandi rurimo itsinda rya methanol hamwe nitsinda rya fosifate ebyiri zidafite aho zibogamiye igice cya sodium.
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5-Bromo-4-chloro-3-indolyl fosifate disodium umunyu CAS: 102185-33-1
5-Bromo-4-chloro-3-indolyl fosifate disodium umunyu (BCIP) ni imiti ivanga imiti ikunze gukoreshwa mubinyabuzima bya molekuline no mubinyabuzima.Nibintu bya chromogenic substrate ya enzymes ya alkaline fosifata.
BCIP ikoreshwa kenshi ifatanije na nitroblue tetrazolium (NBT) nka substrate kugirango tumenye ibikorwa bya fosifata ya alkaline.Iyo BCIP ihinduwe na fosifata ya alkaline fosifata, imiterere yimvura yubururu, ituma umuntu abona amashusho ya enzyme ihari cyangwa ibikorwa.
Uru ruganda ni ingirakamaro cyane mubikorwa nka immunohistochemie, muri Hybridisation, hamwe na enzyme ihuza immunosorbent (ELISAs) kugirango hamenyekane ahari cyangwa bihari biomolecules cyangwa acide nucleic.Imvura yubururu yashizweho na BCIP itanga ikimenyetso kigaragara gifasha mukumenya no gusesengura molekile ziteganijwe mubitegererezo byubushakashatsi.
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Umunyu wa Sodium Umunyu CAS: 139-41-3 Igiciro cyabakora
N, N-Bis (2-hydroxyethyl) umunyu wa sodium ya glycine ni uruganda rwimiti rukoreshwa nkibikoresho byo mu bwoko bwa biohimiki na biofiziki.Ifasha kugumana urwego pH ruhamye mubihe byubushakashatsi, bikagira akamaro mubushakashatsi bwa enzyme, ubushakashatsi bwa poroteyine, umuco w'utugari, hamwe na tekinoroji yo guhuza iburengerazuba.
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4-Aminophthalhydrazide AMPPD CAS: 3682-14-2
4-Aminophthalhydrazide, izwi kandi nka 4-APhH, ni imiti ivangwa na molekile ya C8H8N2O.Ni mubyiciro bya hydrazide ivanze kandi ikomoka kuri acide phthalic.
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N-Acetyl-L-cysteine CAS: 616-91-1
N-Acetyl-L-cysteine (NAC) ni uburyo bwahinduwe bwa sisitemu ya amine aside.Itanga isoko ya sisitemu kandi irashobora guhinduka byoroshye muri tripeptide glutathione, antioxydants ikomeye mumubiri.NAC izwiho kurwanya antioxydeant na mucolytike, ikagira akamaro mubikorwa bitandukanye byubuzima.
Nka antioxydeant, NAC ifasha kurinda selile kwangirika kwatewe na radicals yubusa, ubwoko bwa ogisijeni ikora, nuburozi.Ifasha kandi synthesis glutathione, igira uruhare runini mubikorwa byo kwangiza umubiri no kubungabunga umubiri.
NAC yakozweho ubushakashatsi ku nyungu zishobora kugira mu buzima bw’ubuhumekero, cyane cyane ku bantu bafite ibibazo nka bronhite idakira, COPD, na fibrosis ya cystic.Bikunze gukoreshwa nkibisohoka kugirango bifashe kunanuka no koroshya urusenda, byoroshye guhanagura inzira.
Byongeye kandi, NAC yerekanye amasezerano yo gushyigikira ubuzima bwumwijima ifasha mugukuraho ibintu byuburozi, nka acetaminofeni, igabanya ububabare busanzwe.Irashobora kandi kugira ingaruka zo gukingira kwangirika kwumwijima guterwa no kunywa inzoga.
Usibye imiterere ya antioxydeant nubuhumekero, NAC yashakishijwe kubwinyungu zayo mubuzima bwo mumutwe.Ubushakashatsi bumwe bwerekana ko bushobora kugira ingaruka nziza ku ihungabana ry’imyumvire, nko kwiheba no guhungabana bikabije (OCD).
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Iyode ya Acetylthiocholine CAS: 1866-15-5
Iyode ya Acetylthiocholine ni imiti ikoreshwa cyane nka substrate muri enzyme yerekana gupima ibikorwa bya enzyme acetylcholinesterase (AChE).AChE ni enzyme itanga hydrolyzes ya neurotransmitter acetylcholine, intambwe ikomeye muguhagarika kwanduza ibimenyetso hagati ya selile nervice.
Iyo iyode ya acetylthiocholine ikozwe na AChE, itsinda rya acetyl rirakurwaho, bigatuma habaho ioniyo ya thiocholine na acetate.Thiocholine noneho ikora hamwe na reagent itagira ibara yitwa DTNB (5,5′-dithiobis (2-nitrobenzoic aside)) kugirango ikore ibara ryumuhondo ryitwa 5-thio-2-nitrobenzoate, rishobora gupimwa spekitifotometometrike.Igipimo cyiterambere ryamabara kirahwanye neza nibikorwa bya AChE murugero.
