DNase I, ikomoka kuri Bovine Pancreas CAS:9003-98-9

DNase I, ikomoka kuri pancreas y'inka, ni enzyme igabanya by'umwihariko molekile za ADN binyuze mu gukurura fosiforiste mu mugongo wa ADN. Ingaruka nyamukuru za DNase I ni ukwangirika no gucikamo ibice kwa ADN.

Uburyo bwa mbere DNase I ikoreshwa ni mu bushakashatsi ku binyabuzima bya molekile. Ikunze gukoreshwa mu bushakashatsi butandukanye kugira ngo ivange ADN, ikureho ubwandu bwa ADN mu ngero za RNA, kandi ikore ADN. DNase I igira akamaro cyane mu kwangiza ADN mu buryo bwa poroteyine-DNA, bigatuma abashakashatsi biga imikoranire ya poroteyine-ADN cyangwa gukuraho poroteyine zifatanye na ADN.

Byongeye kandi, DNase I ikunze gukoreshwa mu gusuzuma ADN, bifasha kumenya uturere twa ADN duhujwe na poroteyine runaka. Mu kuvura ADN hakoreshejwe DNase I hanyuma bagasesengura ibice bya ADN bivuyemo, abashakashatsi bashobora kumenya aho poroteyine zihujwe na ADN zihujwe.

DNase I ikoreshwa kandi mu isesengura rya chromatin, aho ifasha mu igogora rya chromatin (DNA na poroteyine zifitanye isano) kugira ngo yige imiterere n'imiterere yayo. Mu gugogora chromatin hamwe na DNase I, abashakashatsi bashobora kumenya uturere tworoshye kandi tworoshye gucikamo ibice, bagatanga ubumenyi ku miterere ya gene n'imiterere ya chromatin.

Byongeye kandi, DNase I ifite akamaro mu gusuzuma indwara, cyane cyane mu gupima no gupima ADN idafite uturemangingo mu maraso atembera. Mu gukoresha DNase I mu gusya ADN idatembera, birashoboka gusesengura no kumenya uruhererekane rwa ADN rujyanye n'indwara cyangwa imiterere mibi y'uturemangingo.