DAPI (4,6-Diamidini-2-phenylindole dihydrochloride) CAS:28718-90-3

DAPI (4,6-Diamidino-2-phenylindole dihydrochloride) ni irangi rikoresha fluorescent rikunze gukoreshwa mu bushakashatsi bw’ibinyabuzima mu kureba ADN. Rifatana n’umuyoboro muto wa ADN ifite imigozi ibiri kandi rigatanga fluorescent y’ubururu iyo rimurikiwe n’urumuri rwa ultraviolet. DAPI ikunze gukoreshwa muri mikroskopi ya fluorescent kugira ngo irangize ingirabuzimafatizo z’uturemangingo, bigatuma iba igikoresho cy’ingirakamaro cyo kwiga imiterere n’imiterere ya ADN mu turemangingo. Rinakoreshwa muri cytometry yo gusesengura ibikubiye muri ADN mu turemangingo ku giti cyatwo no mu bundi buryo bwo kureba no gupima ADN. Kuba DAPI ikunda cyane ADN n’imiterere yayo yihariye ituma iba igikoresho gikoreshwa cyane kandi gikoreshwa cyane mu bushakashatsi ku binyabuzima bya molekile n’uturemangingo.