BCIP-Toluidine)5-Bromo-4-chloro-3-indolylphosphate-p-toluidine umunyu CAS:6578-06-9

BCIP-Toluidine, izwi kandi ku izina rya 5-Bromo-4-chloro-3-indolylphosphate-p-toluidine umunyu, ni ikinyabutabire gikunze gukoreshwa mu bushakashatsi ku binyabuzima bya molekile no mu buvuzi bwa biochimie. Ikoreshwa cyane cyane nk'igice cy'ingenzi mu gusuzuma imisemburo ya immunosorbent (ELISAs) cyangwa uburyo bwo gusiga irangi rya immunohistochemical kugira ngo hamenyekane imikorere ya alkaline phosphatase.

Iyo BCIP-Toluidine igabanijwemo na alkaline phosphatase, ihinduka ibara, bigatuma habaho ubururu n'umuhengeri. Iyi reaction ituma habaho kureba no gupima ibikorwa bya alkaline phosphatase mu bipimo. Ubukana bw'ibara bushobora gupimwa hifashishijwe spectrophotometric kandi bungana neza n'ibikorwa bya enzyme.

Gukoresha BCIP-Toluidine nk'igice cy'umubiri bikunze gukoreshwa cyane cyane mu bikorwa birimo ADN cyangwa RNA, nko muri situ hybridization (ISH) cyangwa Southern and Northern blotting. Bitanga uburyo bwizewe kandi bwihuse bwo kumenya niba hari aside nucleic target mu bipimo.

Gutumiza cyangwa Gusaba Igiciro

Ibisobanuro birambuye ku gicuruzwa

Ibirango by'ibicuruzwa

Ishyirwa mu bikorwa n'ingaruka

BCIP-Toluidine (5-Bromo-4-chloro-3-indolylphosphate-p-toluidine umunyu) ni substrate ikoreshwa mu buhanga bwa molekile mu kumenya niba alkaline phosphatase ikora. Iyo ikoreshejwe nk'substrate, BCIP-Toluidine ihinduka ibara iyo alkaline phosphatase icitsemo ibice, bigatuma habaho ubururu n'umuhengeri. Iyi mpinduka y'ibara ituma abashakashatsi bashobora kubona no gupima alkaline phosphatase mu buryo butandukanye bw'ibizamini.

Uburyo nyamukuru bwo gukoresha BCIP-Toluidine ni mu gusuzuma enzyme-linked immunosorbent assays (ELISAs) no mu buhanga bwo gusiga irangi ry’imyuka. Akenshi ikoreshwa mu kumenya antibodies za alkaline phosphatase cyangwa probes za alkaline phosphatase muri izi assays. BCIP-Toluidine ikoreshwa cyane mu bushakashatsi bwa biology ya molekile mu kureba no gupima imiterere ya gene, aho poroteyine iherereye, n'imikorere ya enzyme.

Gupakira ibicuruzwa:

Amakuru y'inyongera:

Imiterere	C8H6BrClNO4P.C7H9N
Isuzuma	99%
Isura	Ifu y'umweru
Nimero ya CAS	6578-06-9
Gupakira	Bito kandi byinshi
Igihe cyo kuruhuka	Imyaka 2
Ububiko	Bika ahantu hakonje kandi humutse
Icyemezo	ISO.