BCIP-Toluidine)5-Bromo-4-chloro-3-indolylphosphate-p-toluidine umunyu CAS:6578-06-9

Umunyu wa 5-Bromo-4-chloro-3-indolyl phosphate p-toluidine ni ikinyabutabire gikunze gukoreshwa mu bushakashatsi ku binyabuzima bya molekile no mu buvuzi bwa biochimie. Ukunze gukoreshwa mu gusuzuma immunosorbent assays (ELISA) no mu buryo bwo gushyira ibirango bya poroteyine.

Iki kinyabutabire ni substrate ya alkaline phosphatase, enzyme ikoreshwa cyane muri biology ya molekile mu kumenya niba hari molekile cyangwa poroteyine runaka binyuze mu buryo bw'amabara cyangwa chemiluminescent.

Iyo enzyme ya alkaline phosphatase ihari, igabanya amatsinda ya fosfeti mu munyu wa 5-Bromo-4-chloro-3-indolyl phosphate p-toluidine, bigatuma habaho irangi ry'ubururu cyangwa umuhengeri. Iri hinduka ry'ibara rishobora kugaragara no gupimwa byoroshye, bigatuma habaho gupima imikorere ya enzyme cyangwa kuba hari molekile zigamije.

Gutumiza cyangwa Gusaba Igiciro

Ibisobanuro birambuye ku gicuruzwa

Ibirango by'ibicuruzwa

Ishyirwa mu bikorwa n'ingaruka

Western blotting: BCIP ikoreshwa mu gupima no kwiyumvisha poroteyine zimuwe ku ruhu nyuma ya gel electrophoresis. Antibodies za alkaline phosphatase-conjugated secondary zikoreshwa mu gufatana n'izisanzwe, hanyuma BCIP igatera ihinduka ry'ubururu aho poroteyine ziri.

Ubuvuzi bw'imyororokere: BCIP ikoreshwa mu buryo bwo gusiga irangi ry'imyororokere kugira ngo hamenyekane niba hari poroteyine runaka mu turemangingo. Udukoko twa kabiri twa alkaline phosphatase-conjugated antibodies dukoreshwa mu gufatana n'uturemangingo tw'ibanze, naho uburyo bwo kuvura BCIP butanga ibara ry'ubururu iyo enzyme iriho, bigaragaza aho poroteyine iri mu gice cy'umubiri.

Uburyo bwo guhuza ibintu muri situ: BCIP ikoreshwa kandi mu kumenya uruhererekane rw'aside nucleic mu turemangingo cyangwa mu turemangingo tudahinduka. Ibikoresho byo guhuza ibintu bishyirwaho ikimenyetso cya alkaline phosphatase, kandi iyo BCIP yongereweho, kuba hari mRNA cyangwa ADN by'ingenzi bigaragazwa n'ibara ry'ubururu.

Gupakira ibicuruzwa:

Amakuru y'inyongera:

Imiterere	C8H6BrClNO4P.C7H9N
Isuzuma	99%
Isura	Ifu y'umweru
Nimero ya CAS	6578-06-9
Gupakira	Bito kandi byinshi
Igihe cyo kuruhuka	Imyaka 2
Ububiko	Bika ahantu hakonje kandi humutse
Icyemezo	ISO.