Avidin CAS: 1405-69-2 Igiciro cy'umukozi

Gutunganya Poroteyine: Avidin ishobora kubuzwa kugenda hakoreshejwe icyuma gikomeye, nka chromatography resin cyangwa magnetic beads, kandi igakoreshwa mu gufata poroteyine za biotinyle cyangwa aside nucleic. Ubu buryo buzwi nka avidin affinity chromatography, kandi butuma habaho gutunganya molekile runaka mu buryo bwihariye.

Ibikoresho bya Biotinyled: Avidin ikunze guhuzwa na enzymes, fluorophores, cyangwa ibindi birango kandi ikoreshwa nk'ikintu kiyifasha kumenya mu bipimo bitandukanye. Ibikoresho bya Biotinyled, nka antibody cyangwa aside nucleic probes, bishobora kumenyekana hifashishijwe molecules zijyanye na avidin. Ubu buryo bukunze gukoreshwa muri ELISA, immunohistochemistry, na nucleic acid hybridization assays.

Gutanga imiti ku buryo bugamije: Avidin ishobora gukoreshwa nk'ikintu gitwara imiti cyangwa imiti ivura by'umwihariko ku turemangingo cyangwa ingirabuzimafatizo zitanga imiti ya biotin. Mu guhuza biotin n'imiti cyangwa uduce duto tw'imiti no kuyihuza na avidin, hashobora gukorwa uburyo bwo gutanga imiti ku buryo bugamije.

Ubuhanga bwa Molecular Biology: Sisitemu zishingiye kuri Avidin zikoreshwa cyane mu bushakashatsi bwa molecular biology. Urugero, streptavidin, ifite isano nk'iya biotin nka avidin, ikoreshwa mu buryo nka ADN sequencing, PCR, no muri situ hybridization, aho ihuzwa na biotinylated probes cyangwa primers.

Gusuzuma no Gupima: Avidin ishobora gushyirwaho ikimenyetso cya radioisotopes, amarangi ya fluorescent, cyangwa uduce duto tw’ingirabuzimafatizo, bigatuma iba igikoresho cy’ingirakamaro mu buhanga bwo gufata amashusho nka positron emission tomography (PET), fluorescence microscopy, na magnetic resonance imaging (MRI). Byongeye kandi, sisitemu zishingiye kuri avidin zikoreshwa mu bizamini byo gusuzuma, nko gusuzuma immunoassays, kugira ngo hamenyekane niba hari ibimenyetso byihariye by’ingirabuzimafatizo.