5,5-Dithiobis (aside nitrobenzoic 2) CAS: 69-78-3 Ishusho y'ingenzi

5,5-Dithiobis (aside 2-nitrobenzoic) CAS: 69-78-3

5,5-Dithiobis (aside 2-nitrobenzoic), izwi kandi nka DTNB cyangwa Ellman's reagent, ni ikinyabutabire gikunze gukoreshwa mu bushakashatsi ku binyabutabire n'imiti. Ni ifu y'umuhondo, ikoze nk'iy'amabara atukura kandi ishongesha cyane mu mazi no mu bintu bishongesha mu buryo bw'umwimerere.

DTNB ikoreshwa cyane cyane mu gupima ingano y'amatsinda ya sulfhydryl (-SH) muri poroteyine na peptides. Iki gikoresho gihura na thiols, bigatuma habaho disulfide ivanze hanyuma hagasohoka 2-nitro-5-thiobenzoate, ifite ibara ry'umuhondo rishobora gupimwa hifashishijwe spectrophotometric. Mu gupima ubukana bw'ibara ry'umuhondo, birashoboka gupima ingano y'amatsinda ya thiols mu rugero. Ubu buryo bukunze gukoreshwa mu kwiga imikoranire ya poroteyine na poroteyine, gupfunyika kwa poroteyine, n'imikorere ya enzymes ihuriweho n'amatsinda ya thiol.

Uretse gupima thiol, DTNB yakoreshejwe mu zindi ngamba, nko gusuzuma imiterere ya redox y'uturemangingo, kwiga uburyo ibintu bifatanye na metal-sulfure bihagaze, no gupima imikorere ya enzymes zitandukanye.

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Ishyirwa mu bikorwa n'ingaruka

Ingano ya Thiol: DTNB ikoreshwa cyane mu gusuzuma ingano ya thiols mu bipimo bya poroteyine. Aya makuru ni ingenzi mu kwiga imikoranire ya poroteyine na poroteyine, gupfunyika kwa poroteyine, n'imikorere ya enzymes mu matsinda ya thiol. Igisubizo cya colorimetric cya DTNB cyemerera gupima thiols binyuze mu bipimo bya spectrophotometric.

Isuzuma rya Redox State: DTNB yakoreshejwe mu gusuzuma imiterere ya redox mu turemangingo, mu ngingo, cyangwa mu binyabuzima. Mu gupima impinduka mu gipimo cya thiol, abashakashatsi bashobora kubona ubumenyi ku bijyanye n'ihungabana rya oxidative, imikorere ya antioxidant, n'inzira zo gutanga ibimenyetso bya redox.

Isuzuma rya Enzyme: Uburyo DTNB ikora neza hamwe na thiols butuma iba igikoresho cy'agaciro mu isuzuma rya enzymes. Enzymes nyinshi, nka glutathione-S-transferases, cystathionine-γ-lyase, na thioredoxin reductase, zishingiye kuri thiol kandi zishobora gupimwa cyangwa gupimwa hakoreshejwe DTNB.

Isesengura ry’Uburimbane bw’Ibinyabutabire by’Ibyuma n’Isulfure: DTNB ikoreshwa mu gusuzuma uburimbane n’imikorere y’ibinyabutabire by’Ibyuma n’Isulfure na poroteyine za metallo. Ibinyabutabire bishobora kumenya no gupima niba hari ibinyabutabire bigira uruhare mu ihindagurika ry’ibinyabutabire by’Ibyuma, bigatanga amakuru y’ingenzi ku mikoranire y’ibinyabutabire by’Isulfure n’imibanire y’ibinyabutabire.

Gupakira ibicuruzwa:

Amakuru y'inyongera:

Imiterere	C14H8N2O8S2
Isuzuma	99%
Isura	Igiti cy'umuhondo gitukura
Nimero ya CAS	69-78-3
Gupakira	Bito kandi byinshi
Igihe cyo kuruhuka	Imyaka 2
Ububiko	Bika ahantu hakonje kandi humutse
Icyemezo	ISO.