5-Bromo-4-chloro-3-indolyl-N-acetyl-beta-D-glucosaminide CAS: 4264-82-8

5-Bromo-4-chloro-3-indolyl-N-acetyl-beta-D-glucosaminide nuruvange rukoreshwa mubushakashatsi butandukanye bwibinyabuzima, cyane cyane mugushakisha no kubona amashusho yibikorwa bya enzyme.Ni substrate ishobora kuba hydrolyz na enzymes zihariye, bikavamo kurekura ibicuruzwa byamabara cyangwa fluorescent.

Uru ruganda rusanzwe rukoreshwa mubushakashatsi kugirango hamenyekane ahari nibikorwa bya enzymes nka beta-galactosidase na beta-glucuronidase.Iyi misemburo ikuramo amatsinda ya acetyl na glucosaminide kuva muri substrate, biganisha kuri chromofore yubururu cyangwa icyatsi.

Imiterere yihariye ya 5-Bromo-4-chloro-3-indolyl-N-acetyl-beta-D-glucosaminide itanga uburyo bworoshye bwo kumenya no kugereranya ibikorwa bya enzyme.Gukoresha muburyo butandukanye bwubushakashatsi, harimo histochemie, immunohistochemie, hamwe nubushakashatsi bushingiye ku ngirabuzimafatizo, byagize uruhare mu gusobanukirwa neza imikorere ya enzyme.

Tegeka cyangwa usabe amagambo

Ibicuruzwa birambuye

Ibicuruzwa

Gushyira mu bikorwa n'ingaruka

5-Bromo-4-chloro-3-indolyl-N-acetyl-beta-D-glucosaminide (X-Gluc) ni insimburangingo ya chromogenic ikunze gukoreshwa mu kumenya ibikorwa bya beta-glucuronidase (GUS).GUS ni enzyme iboneka mu binyabuzima bitandukanye, harimo bagiteri, ibimera, n’inyamabere.X-Gluc ikoreshwa kenshi mubanyamakuru ba GUS hamwe nubushakashatsi bwibinyabuzima bya molekuline.

Porogaramu nyamukuru ya X-Gluc iri mubuhanga bwo gusiga amateka ya histochemiki, aho ishobora kwiyumvisha imvugo nibikorwa bya enzyme ya GUS.Iyi substrate ni selile-yinjira kandi ihinduka hydrolyzed na GUS, bigatuma habaho imvura yubururu cyangwa ibicuruzwa bidashonga.Iri bara ry'ubururu rifasha abashakashatsi kumenya no kumenyekanisha ibikorwa bya GUS mu ngirabuzimafatizo, ingirangingo, n'ibinyabuzima byose.

X-Gluc irashobora kandi gukoreshwa muburyo bwo gupima ibikorwa bya GUS enzyme.Ubwinshi bwamabara yubururu cyangwa ingano yibicuruzwa byakozwe birashobora guhuzwa nurwego rwimvugo ya GUS cyangwa ibikorwa byayo bitera imbaraga.

Byongeye kandi, X-Gluc yakoreshejwe mubushakashatsi bwerekeranye nubwoko bwibimera kugirango yige imvugo ya gene, ibikorwa byabateza imbere, no guhindura ibimera.Yakoreshejwe kandi muri sisitemu ya bagiteri yo gukoroniza no kumenya poroteyine za GUS fusion.

Gupakira ibicuruzwa:

Amakuru yinyongera:

Ibigize	C16H18BrClN2O6
Suzuma	99%
Kugaragara	Ifu yera
URUBANZA No.	4264-82-8
Gupakira	Ntoya kandi nini
Ubuzima bwa Shelf	Imyaka 2
Ububiko	Ubike ahantu hakonje kandi humye
Icyemezo	ISO.