4-Nitrophenyl-beta-D-glucopyranoside CAS: 2492-87-7

Enzyme yibikorwa: hydrolysis ya 4-Nitrophenyl-beta-D-glucopyranoside na enzymes nka β-glucosidase irashobora gukoreshwa mugupima ibikorwa bya enzyme.Isohora rya 4-nitropenol irashobora kugereranywa ukoresheje spekitifotometometrie, bigatuma habaho kugena enzyme kinetics hamwe nubushakashatsi bwo kubuza.

Imvugo ya gene hamwe numunyamakuru avuga: 4-Nitrophenyl-beta-D-glucopyranoside irashobora guhuzwa hamwe na tagi ya molekile yihariye yinjizwa muri poroteyine zo guhuza.Igikorwa cya enzyme ya proteine ​​ya fusion irashobora kugereranywa byoroshye mugupima irekurwa rya 4-nitropenol, bigatanga ubushishozi mumagambo ya gene hamwe na protein-proteyine.

Kuvumbura ibiyobyabwenge no gusuzuma byinshi: Hydrolysis ya 4-Nitrophenyl-beta-D-glucopyranoside irashobora gukoreshwa nkigikorwa cya surrogate mugikorwa cyo kuvumbura ibiyobyabwenge.Yemerera kugenzura byihuse amasomero manini yububiko kugirango amenye ibishobora kubuza enzyme cyangwa gukora.

Kwipimisha kwisuzumisha: Indwara zimwe zijyanye no guhindura mubikorwa bimwe na bimwe bya enzyme.Ukoresheje hydrolysis ya 4-Nitrophenyl-beta-D-glucopyranoside na enzymes yihariye nk'ikizamini cyo gusuzuma, izi ndwara zirashobora gutahurwa cyangwa kugenzurwa.Ibi bikubiyemo ibintu bijyanye nuburwayi bwa lysosomal nindwara za hepatobiliary.