4-NITROPHENYL-ALPHA-D-MANNOPYRANOSIDE URUBANZA: 10357-27-4

Enzyme substrates: 4NPM irashobora gukoreshwa nka substrate yimisemburo itandukanye, harimo glycosidase na enzymes zijyanye nayo.Iyi misemburo ikuraho glycosidique ihuza mannose na 4NPM, bigatuma irekurwa rya nitrophenyl.Ingano ya hydrolysis ya substrate irashobora gupimwa spekitifotometometrike mugukurikirana iyinjizwa ryitsinda rya nitrophenyl irekuwe kumuraba wihariye.Ibi bifasha abashakashatsi gusuzuma ibikorwa bya enzyme na kinetics.

Isuzuma rya metabolisme ya karubone: Ukoresheje 4NPM nka substrate, abashakashatsi barashobora kwiga ibikorwa byimisemburo igira uruhare muri metabolisme ya karubone, nka alpha-mannosidase.Iyi misemburo hydrolyze glycosidic ihuza ibice birimo mannose, kandi ibikorwa byayo birashobora gupimwa mugukurikirana irekurwa rya nitrophenyl kuva 4NPM.

Ubushakashatsi bwa Glycosylation: 4NPM irashobora kandi gukoreshwa mugusuzuma iperereza ryimisemburo igira uruhare muri glycosylation.Glycosylation ni inzira yo guhuza molekile ya sukari kuri poroteyine cyangwa izindi molekile, kandi imisemburo myinshi igira uruhare muri iki gikorwa.Mugukoresha 4NPM nkibikoresho byakira, abashakashatsi barashobora gupima ihererekanyabubasha ryisukari kuri 4NPM na enzymes zihariye zigira uruhare muri glycosylation.

Kugenzura inzitizi ya enzyme cyangwa abayikora: 4NPM irashobora gukoreshwa murwego rwohejuru rwerekana ibizamini kugirango hamenyekane ibice bibuza cyangwa bikora imisemburo yihariye.Mugusuzuma ingaruka ziterwa nibizamini kuri hydrolysis cyangwa guhindura 4NPM na enzymes zigenewe, abashakashatsi barashobora kumenya imiti ishobora kuvura cyangwa imiti yingirakamaro yo kwiga imikorere ya enzyme.