4-Nitrophenyl-2-acetamido-2-deoxy-β-D-glucopyranoside CAS:3459-18-5

Igice cya Enzyme: pNAG ikoreshwa cyane nk'igice cyihariye cya enzymes zitandukanye zigira uruhare mu ikorwa rya hydrolysis y'imigozi ya β-D-glucoside. Iyo izi enzymes ziciyemo molekile ya pNAG, isohora p-nitrophenol. Ibi bituma abashakashatsi bashobora gupima no gupima imikorere ya enzymes.

Isuzuma ry'ibikorwa bya enzyme: Uburyo enzymes zikoresha hydrolysis ya pNAG zishobora kumenyekana no gupimwa hifashishijwe spectrophotometric. Ibi bituma pNAG ikwiriye gukoreshwa mu isuzuma ry'ibikorwa bya enzyme, aho ingano ya p-nitrophenol ikorwa ingana neza n'imikorere ya enzyme.

Gupima Umuvuduko Ukabije: pNAG ikoreshwa cyane mu gusuzuma umuvuduko mwinshi kugira ngo hamenyekane kandi hamenyekane uburyo enzyme ibuza cyangwa ikora. Mu gusuzuma ingaruka z'ibintu bitandukanye ku gikorwa cya enzyme, abashakashatsi bashobora kumenya imiti ishobora kuba cyangwa ibintu bishobora guhindura imikorere ya enzyme.

Ubushakashatsi ku igaragara ry’uturemangingo: pNAG ikoreshwa kandi mu bushakashatsi ku binyabuzima bya molekile kugira ngo yige igaragara ry’uturemangingo n’uburyo tugenzurwa. Mu gupima imikorere ya enzymes za enzymes runaka hakoreshejwe pNAG nk'igice cy’ingenzi, abashakashatsi bashobora gusuzuma ingaruka z’igaragara ry’uturemangingo ku mikorere n’imikorere ya enzymes.