4-Nitrophenyl-2-acetamido-2-deoxy-β-D-glucopyranoside CAS: 3459-18-5

Enzyme Substrate: pNAG ikoreshwa cyane nka substrate yihariye ya enzymes zitandukanye zigira uruhare muri hydrolysis ya bond-D-glucoside.Iyo iyo misemburo ikuraho molekile ya pNAG, irekura p-nitropenol.Ibi bituma abashakashatsi gupima no kugereranya ibikorwa byimikorere.

Igikorwa cya Enzyme Isuzuma: Hydrolysis ya pNAG na enzymes yihariye irashobora gutahurwa no gupimwa spekitifotometometricique.Ibi bituma pNAG ibereye ibikorwa bya enzyme isuzuma, aho ingano ya p-nitropenol yakozwe iba ihuye neza nigikorwa cyimisemburo.

Kugenzura Byinshi-Kwinjiza: pNAG isanzwe ikoreshwa murwego rwohejuru rwerekana ibicuruzwa kugirango tumenye kandi biranga enzyme inhibitor cyangwa abayikora.Mugusuzuma ingaruka ziterwa nibintu bitandukanye mubikorwa bya enzymatique, abashakashatsi barashobora kumenya abakandida ibiyobyabwenge cyangwa abayobora imikorere ya enzyme.

Ubushakashatsi bwerekana Gene: pNAG nayo ikoreshwa mubushakashatsi bwibinyabuzima bwa molekuline kugirango bige imvugo ya gene.Mugupima ibikorwa byimisemburo yimisemburo yihariye ukoresheje pNAG nka substrate, abashakashatsi barashobora gukora iperereza ku ngaruka zerekana imiterere ya gene kumikorere ya enzyme.