3,3′-Diaminobenzidine CAS:91-95-2

Ingaruka za 3,3'-Diaminobenzidine (DAB) ni ukugira ibara ry'umukara iyo ihuye na enzymes za peroxidase. Iri hinduka ry'ibara rituma habaho ishusho ya antigens cyangwa biomolecules zishishikajwe na immunohistochemistry (IHC) na immunocytochemistry (ICC). DAB ikunze gukoreshwa mu bushakashatsi bwa biomedical no mu gusuzuma kugira ngo hamenyekane kandi hamenyekane antigens cyangwa poroteyine runaka mu bipimo by'ibinyabuzima. Mu kongeramo DAB nk'igice cy'umubiri hanyuma hagakurikiraho reaction hamwe na enzymes za peroxidase, havuka umwuka w'umukara aho antigens zigomba gukorerwa. Gukoresha DAB irangi ni ingirakamaro cyane mu kwiga indwara n'imiterere itandukanye. Bifasha abashakashatsi n'abahanga mu by'indwara kwiyumvisha no gusesengura ikwirakwira, ubwinshi, n'aho biomolecules runaka ziherereye mu turemangingo cyangwa mu turemangingo. Aya makuru afasha mu gusobanukirwa uburyo indwara ziterwa, kwiga inzira z’uturemangingo, no kumenya intego zishobora guterwa no gusuzuma cyangwa kuvura. Byongeye kandi, gusiga irangi rya DAB bishobora gukoreshwa mu buryo butandukanye bwa molekile kugira ngo harebwe uburyo poroteyine n’iy’uturemangingo bikorana, hamenyekane imiterere y’igaragaza rya poroteyine, hamenyekane niba hari ibimenyetso byihariye, kandi hasuzumwe uburyo bwo kuvura bukora neza. Bitewe n’uko gusiga irangi ryizewe kandi rikomeye, ndetse no guhuza n’uburyo butandukanye bwo gupima no gukoresha mikorosikope, DAB yabaye substrate ikoreshwa cyane mu gusuzuma indwara za immunohistochemistry na immunocytochemistry.