3,3,5,5 -Tetramethylbenzidine dihydrochloride hydrate CAS:207738-08-7

3,3', 5,5'-Tetramethylbenzidine dihydrochloride (TMB) ni substrate ikoreshwa cyane mu gusuzuma immunosorbent assays (ELISAs) n'izindi assays za biochemical. Iyo TMB ikoreshejwe oxidized na enzyme horseradish peroxidase (HRP) mu gihe hari hydrogen peroxide, itanga ibara ry'ubururu. Iri hinduka ry'ibara rikoreshwa mu kumenya niba hari antibodies za HRP cyangwa izindi poroteyine, akenshi bigaragaza ko hari antigen runaka mu gipimo. Ibara ry'ubururu rikomoka ku gikorwa gikunze gupimwa hakoreshejwe spectrophotometer, ituma hapimwa ubukana bw'ihinduka ry'ibara kandi igatanga uburyo bwo guhuza ubukana bw'ibara n'ingano ya molecule igamije iri mu gipimo. TMB ifite uburyo bwinshi bwo gusuzuma, ubushakashatsi, no guteza imbere imiti, cyane cyane mu kumenya antibodies, imisemburo, cytokines, n'izindi biomolecules. Uburyo ikora, ihamye, no koroshya ikoreshwa ryayo bituma iba igikoresho cy'agaciro mu buryo butandukanye bwo gusuzuma no gupima molecules runaka.