3-NITROPHENYL-BETA-D-GALACTOPYRANOSIDE URUBANZA: 3150-25-2

3-Nitrophenyl-beta-D-galactopyranoside (ONPG) ni substrate ikunze gukoreshwa mubushakashatsi bwimisemburo kugirango tumenye kandi dupime ibikorwa bya beta-galactosidase.Iyo beta-galactosidase ihari kandi ikora, itanga hydrolyzes ONPG, ikarekura ibicuruzwa byamabara yumuhondo bita 3-nitropenol.Ubwinshi bwamabara yumuhondo yakozwe burashobora gupimwa spekitifotometometrike, bigatuma umubare wibikorwa bya beta-galactosidase.ONPG ikoreshwa cyane mubinyabuzima bwa molekuline no mubushakashatsi bwa mikorobi, ndetse no mugupima kwa muganga, kugirango bige imvugo ya gene, imikorere ya poroteyine, kumenya bagiteri, hamwe nubuzima bwa selile.

Tegeka cyangwa usabe amagambo

Ibicuruzwa birambuye

Ibicuruzwa

Gushyira mu bikorwa n'ingaruka

Kumenya ibikorwa bya beta-galactosidase: ONPG ikoreshwa kenshi kugirango hamenyekane niba ibikorwa bya beta-galactosidase bihari mubikorwa bitandukanye byibinyabuzima, nkumuco wa bagiteri cyangwa lysates.Umusaruro wa o-nitropenol, ufite ibara ry'umuhondo, urashobora gupimwa byoroshye ukoresheje spekitifotometero.

Ubushakashatsi bwerekana imvugo: ONPG ikoreshwa mubushakashatsi bwibinyabuzima bwa molekuline kugirango bige imvugo ya gene.Muguhuza porotokoro ya gene yinyungu hamwe na gene irimo beta-galactosidase, abashakashatsi barashobora gupima ibikorwa byu muterankunga wongeyeho ONPG no kugereranya umusaruro wa o-nitropenol.Ubu buryo, buzwi nka beta-galactosidase umunyamakuru asay, butanga amakuru ajyanye nigikorwa cyo kwandukura gene.

Kumenyekanisha kwa bagiteri: Bagiteri zimwe zitanga beta-galactosidase, mugihe izindi zitabikora.ONPG irashobora gukoreshwa ifatanije nibindi bizamini bya biohimiki kugirango imenye amoko ya bagiteri ukurikije ubushobozi bwa hydrolyze ONPG.Ubu buryo bukunze gukoreshwa muri laboratoire yo kwisuzumisha no muri laboratoire ya mikorobi.

Kugenzura enzyme inhibitor cyangwa abayikora: ONPG irashobora gukoreshwa mugusuzuma ibice bigabanya ibikorwa bya beta-galactosidase.Mugupima ibikorwa bya enzyme imbere yibintu bitandukanye, abashakashatsi barashobora kumenya ibishobora kubuza cyangwa gukora ibikorwa bishobora gukorwaho iperereza kubushobozi bwabo bwo kuvura.

Gupakira ibicuruzwa:

Amakuru yinyongera:

Ibigize	C12H15NO8
Suzuma	99%
Kugaragara	Cyeraifu
URUBANZA No.	3150-25-2
Gupakira	Ntoya kandi nini
Ubuzima bwa Shelf	Imyaka 2
Ububiko	Ubike ahantu hakonje kandi humye
Icyemezo	ISO.