3-[(3-Cholanidopropyl)dimethylammonio]-1-propanesulfonate CAS:75621-03-3

CHAPS (3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate) ni isabune ikoreshwa cyane muri biochimie na molekile. Ni isabune ya zwitterionic, bivuze ko ifite itsinda ry’isabune nziza n’iry’isabune mbi.

CHAPS izwiho ubushobozi bwo gushonga no gushimangira poroteyine zo mu rukenyerero, bigatuma igira akamaro mu bikorwa bitandukanye nko gukuramo poroteyine, kuyisukura no kuyigereranya. Ibangamira imikoranire ya lipide na poroteyine, bigatuma poroteyine zo mu rukenyerero zikurwa mu buryo bwazo.

Bitandukanye n'izindi sabune, CHAPS ni nziza cyane kandi ntigabanya poroteyine nyinshi, bityo ikaba amahitamo meza yo kubungabunga imiterere n'imikorere ya poroteyine mu gihe cy'igerageza. Ishobora kandi gufasha gukumira kwivanga kwa poroteyine.

CHAPS ikoreshwa cyane mu buryo nka SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis), isoelectric focusing, na Western blotting. Ikoreshwa kandi mu bushakashatsi bujyanye na enzymes ziboshye mu membrane, transduction y'ibimenyetso, n'imikoranire ya poroteyine na lipid.

Gutumiza cyangwa Gusaba Igiciro

Ibisobanuro birambuye ku gicuruzwa

Ibirango by'ibicuruzwa

Ishyirwa mu bikorwa n'ingaruka

Gukuramo poroteyine: CHAPS ikoreshwa cyane mu gukuramo poroteyine zo mu ruhu mu bipimo by'ibinyabuzima. Ifasha gushonga kw'izo poroteyine no kubungabunga imiterere yazo.

Gutunganya poroteyine: CHAPS ikoreshwa mu buryo butandukanye bwo gutunganya poroteyine, nko gukoresha chromatography y’ubudahangarwa. Ishobora kongerwa mu bikoresho byo gutunganya poroteyine kugira ngo ishonge kandi ihamye mu gihe cyo gutunganya poroteyine.

Kugaragaza Poroteyine: CHAPS ikunze gukoreshwa mu bushakashatsi bujyanye no kumenya imiterere ya poroteyine zo mu rukenyerero. Ifasha mu kubungabunga imiterere ya poroteyine n'imikorere yayo mu gihe cy'igerageza nko gusuzuma ibikorwa bya enzyme, gukorana kwa poroteyine na poroteyine, no gusesengura spectroscopic.

Ubushakashatsi ku poroteyine y'ururabo: Poroteyine y'ururabo igira uruhare runini mu mikorere myinshi y'uturemangingo. CHAPS ikunze gukoreshwa mu bushakashatsi bujyanye no gutanga ibimenyetso, imikorere y'inzira ya iyoni, imikoranire ya poroteyine n'amavuta, no gutuma poroteyine ikora neza.

Electrophoresis: CHAPS ikoreshwa mu buryo nka SDS-PAGE na isoelectric yibanda ku gushonga kwa poroteyine zo mu ruhu no koroshya gutandukana no gusesengura kwazo.

Gupakira ibicuruzwa:

Amakuru y'inyongera:

Imiterere	C32H58N2O7S
Isuzuma	99%
Isura	Ifu y'umweru ya kristali
Nimero ya CAS	75621-03-3
Gupakira	Bito kandi byinshi
Igihe cyo kuruhuka	Imyaka 2
Ububiko	Bika ahantu hakonje kandi humutse
Icyemezo	ISO.