2-NITROPHENYL-BETA-D-GLUCOPYRANOSIDE URUBANZA: 2816-24-2

2-Nitrophenyl-beta-D-glucopyranoside ni imiti igizwe na molekile ya glucopyranoside ifatanye na nitrophenyl.Bikunze gukoreshwa nka substrate mubisubizo byimikorere kugirango tumenye kandi tugereranye ibikorwa byimisemburo nka beta-glucosidase.Itsinda rya nitrophenyl rishobora gutoborwa na enzyme, bikavamo kurekura ibicuruzwa byamabara yumuhondo bishobora gupimwa spekitifoto.Uru ruganda ni ingirakamaro cyane mukwiga enzyme kinetics hamwe no kwinjiza-hejuru cyane ya enzyme inhibitor cyangwa abayikora.Ikoreshwa kandi mubushakashatsi bwibinyabuzima kugirango hakorwe iperereza kuri metabolisme ya karubone kandi nka glycosidic-ihuza-substrate yihariye.

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Ibicuruzwa

Gushyira mu bikorwa n'ingaruka

Enzyme substrate: ONPG isanzwe ikoreshwa nka substrate ya beta-galactosidase, enzyme hydrolyzes ONPG kugirango itange ibara ryumuhondo (o-nitrophenol) rishobora kumenyekana byoroshye kuri spekitifoto.Iyi reaction enzymatique irashobora gukoreshwa mugupima ibikorwa bya beta-galactosidase, bigatuma ONPG igikoresho cyingirakamaro mubushakashatsi bwa enzymologiya.

Ibinyabuzima bya molekuline bisobanura: ONPG ikoreshwa nka substrate mubushakashatsi butandukanye bwibinyabuzima, cyane cyane muri beta-galactosidase umunyamakuru gene asuzuma.Muri ubu bushakashatsi, insimburangingo ishingiye kuri ONPG ikoreshwa mu gupima ibikorwa by’umunyamakuru gene, ubusanzwe igengwa n’urwego runaka rwamamaza inyungu.Igikorwa cya beta-galactosidase, cyerekanwa nimpinduka yamabara yakozwe kuri hydrolysis ya ONPG, itanga amakuru yingirakamaro kubikorwa byabateza imbere.

Isesengura ryerekana imiterere: ONPG nayo ikoreshwa mugusesengura imvugo ya gene.Muguhuza poroteri ikurikirana yinyungu na beta-galactosidase, abashakashatsi barashobora gupima ibikorwa bya beta-galactosidase bakoresheje ONPG nka substrate.Urwego rwibikorwa bya beta-galactosidase rugaragaza imbaraga nigikorwa cya promoteri, bigatuma hasuzumwa urwego rwerekana imiterere ya gene.

Porogaramu yo gusuzuma: ONPG irashobora gukoreshwa mubikorwa byo gusuzuma, cyane cyane mugutahura mikorobe zitera indwara.Bagiteri zitandukanye, nka Escherichia coli nubwoko bumwe na bumwe bwa Shigella na Salmonella, zitanga beta-galactosidase ishobora gukuramo ONPG.Iyi hydrolysis reaction itanga amabara agaragara, ashobora gukoreshwa kugirango hamenyekane ko izo bagiteri zihari mubitaro byubuvuzi.

Icyitegererezo cyibicuruzwa

Gupakira ibicuruzwa:

Amakuru yinyongera:

Ibigize	C12H15NO8
Suzuma	99%
Kugaragara	Ifu yumuhondo yoroheje
URUBANZA No.	2816-24-2
Gupakira	Ntoya kandi nini
Ubuzima bwa Shelf	Imyaka 2
Ububiko	Ubike ahantu hakonje kandi humye
Icyemezo	ISO.