Phenyl2,3,4,6-tetra-O-acetyl-1-thio-β-D-galactopyranoside CAS:24404-53-3

Phenyl2,3,4,6-tetra-O-acetyl-1-thio-β-D-galactopyranoside ikunze gukoreshwa nk'umusemburo w'umusemburo wa β-galactosidase. Iyo β-galactosidase ikoze kuri yo, icyo kintu gishyirwamo amazi, bigatuma habaho p-nitrophenol cyangwa o-nitrophenol, bifite ibara ry'umuhondo rishobora kugaragara byoroshye. Ibi bituma kiba ingirakamaro nk'umusemburo w'ibizamini bipima imikorere ya β-galactosidase.

Uburyo bumwe bukunze gukoreshwa bwa Phenyl2,3,4,6-tetra-O-acetyl-1-thio-β-D-galactopyranoside ni mu isuzuma rya gene y’umunyamakuru. Muri izi suzuma, gene ishimishije ihuzwa na gene y’umunyamakuru, nka gene ya beta-galactosidase. Hanyuma imikorere ya gene y’umunyamakuru ikurikiranwa hapimwa imikorere ya β-galactosidase hakoreshejwe Phenyl2,3,4,6-tetra-O-acetyl-1-thio-β-D-galactopyranoside nk'igice cy'ingenzi. Ibi bituma abashakashatsi biga uburyo gene ikoreshwa n'uburyo ikoreshwa.

Phenyl2,3,4,6-tetra-O-acetyl-1-thio-β-D-galactopyranoside ishobora kandi gukoreshwa mu gusuzuma inzitizi cyangwa ibikorwa bya β-galactosidase. Mu gupima ibintu bitandukanye cyangwa imiti ishobora gukoreshwa, abashakashatsi bashobora kumenya molekile zihindura imikorere y'iyi enzyme, zishobora kugira akamaro mu kuvura indwara zimwe na zimwe cyangwa indwara zimwe na zimwe.

Byongeye kandi, iyi mvange ishobora gukoreshwa mu gutunganya poroteyine. β-galactosidase ikunze guhuzwa na poroteyine zikenewe kugira ngo zorohereze kuzimenya no kuzitunganya. Phenyl2,3,4,6-tetra-O-acetyl-1-thio-β-D-galactopyranoside ishobora gukoreshwa nk'ifatizo ryo gusuzuma no gusukura izi poroteyine zivanze binyuze mu kumenya imikorere ya β-galactosidase.