Nigute ushobora kumenya icyerekezo cyiza?
Kuri inducer IPTG (isopropyl-beta-d-thiogalactoside), uko kwibanda cyane, nibyiza.Ibyiza byo kwibandaho biterwa nuburyo bwihariye bwo kugerageza ningaruka zifuzwa.
Mubisanzwe, kwibanda kwa IPTG bikoreshwa murwego rwa 0.1-1 mM.Kwibanda cyane birashobora kugabanya ingaruka mbi kumikurire ya selile kandi birashobora kugabanya cytotoxicité bitewe no gukabya gukabije kwa poroteyine.Kwibanda cyane birashobora gutera uburemere bwimikorere ya selile, bikagira ingaruka kumikurire no gukora neza.
Inzira yo kumenya icyerekezo cyiza irashobora kuba ugusuzuma urwego rwimiterere ya poroteyine igamije gukora ibizamini bya induction ya IPTG mubitekerezo bitandukanye.Ibizamini byumuco muto birashobora gukorwa hifashishijwe urutonde rwa IPTG (urugero: 0.1 mM, 0.5 mM, 1 mM, nibindi) kandi ingaruka zigaragaza mubitekerezo bitandukanye zishobora gusuzumwa no kumenya urwego rwerekana poroteyine (urugero: Iburengerazuba blot cyangwa fluorescence detection).Ukurikije ibisubizo byubushakashatsi, kwibanda hamwe ningaruka nziza zerekana imvugo byatoranijwe nkibintu byiza.
Wongeyeho, urashobora kandi kwifashisha ibitabo bijyanye cyangwa uburambe bwizindi laboratoire kugirango usobanukirwe na IPTG ikunze gukoreshwa mugihe cyibigeragezo bisa, hanyuma ugahindura kandi ugahindura ukurikije ibikenewe mubushakashatsi.
Ni ngombwa kumenya ko kwibandaho kwiza gushobora gutandukana bitewe na sisitemu zitandukanye zerekana imvugo, intego za poroteyine, hamwe nubushakashatsi bwakozwe, nibyiza rero guhitamo neza kuri buri kibazo.
Igihe cyo kohereza: Nzeri-28-2023