IPTG CAS: 367-93-1 Igiciro cyabakora
Isopropyl β-D-1-thiogalactopyranoside (IPTG) ni ikigereranyo cya synthique ya lactose ikoreshwa cyane mubushakashatsi bwibinyabuzima bya molekuline no gukoresha ibinyabuzima.IPTG ikoreshwa cyane cyane mugukangura imiterere ya gen muri sisitemu ya bagiteri, aho ikora nka molekuline yo gutangiza iyandikwa rya gen.
Iyo wongeyeho uburyo bwo gukura, IPTG ifatwa na bagiteri kandi irashobora guhuza na proteine ya lac repressor, ikabuza guhagarika ibikorwa bya lac operon.Lac operon ni ihuriro rya gen zigira uruhare muri metabolism ya lactose, kandi iyo poroteyine ya repressor ikuweho, gen zigaragazwa.
IPTG ikoreshwa kenshi ifatanije na lacUV5 mutant promoter, ikaba ari verisiyo ikora ya lac promoter.Muguhuza induction ya IPTG niyi porotokoro ya mutant, abashakashatsi barashobora kugera ku rwego rwo hejuru rwo kwerekana imvugo.Ibi bituma habaho umusaruro mwinshi wa poroteyine yo kwezwa cyangwa izindi porogaramu zo hasi.
Usibye imvugo ya gene, IPTG nayo ikoreshwa kenshi mubururu / bwera bwerekana.Muri ubu buhanga, gene ya lacZ isanzwe ihujwe na gene yinyungu, na bagiteri zigaragaza neza iyi gene ya fusion izatanga enzyme β-galactosidase ikora.Iyo IPTG yongeyeho hamwe na chromogenic substrate nka X-gal, bagiteri zerekana gene fusion ihinduka ubururu kubera ibikorwa bya gal-galactosidase.Ibi bituma habaho kumenya no guhitamo imiterere ya recombinant yahujije neza gene yinyungu.
Kwinjiza imvugo ya gene: IPTG ikoreshwa muburyo bwo kwerekana imiterere ya gen zigenewe muri sisitemu ya bagiteri.Yigana lactose ya inducer naturel kandi ihuza na proteine ya lac repressor, ikayirinda guhagarika lac operon.Ibi bituma habaho kwandukura no kwerekana genes zifuzwa.
Imvugo ya poroteyine no kwezwa: Kwinjiza IPTG akenshi bikoreshwa mu gutanga proteine nyinshi za recombinant ku mpamvu zitandukanye, nk'ubushakashatsi bw’ibinyabuzima, umusaruro uvura, cyangwa isesengura ry’imiterere.Ukoresheje imvugo ikwiye hamwe na IPTG induction, abashakashatsi barashobora kugera ku rwego rwo hejuru rwo gukora poroteyine zigenewe muri bagiteri.
Kugaragaza ubururu / umweru: IPTG ikoreshwa kenshi muguhuza gene ya lacZ hamwe na chromogenic substrate, nka X-gal, kubururu / bwera bwerekana.Gene ya lacZ isanzwe ihujwe na gene yinyungu, na bagiteri zigaragaza neza iyi gene ya fusion izabyara enzyme ya galaktosidase ikora.Iyo IPTG hamwe na chromogenic substrate yongeyeho, imirongo ya recombinant yerekana fusion gene ihinduka ubururu, bigatuma kumenyekana no guhitamo byoroshye.
Kwiga kugenga gene: Induction ya IPTG ikoreshwa mubushakashatsi bwo kwiga amabwiriza ya gen na operons, cyane cyane lac operon.Mugukoresha imbaraga za IPTG no kugenzura imvugo yibigize lac operon, abashakashatsi barashobora gukora iperereza kuburyo bwo kugenzura gene nuruhare rwibintu bitandukanye cyangwa ihinduka.
Sisitemu yo kwerekana imiterere: IPTG nigice cyingenzi muri sisitemu nyinshi zerekana imiterere ya gene, nka sisitemu ya T7 yamamaza.Muri ubu buryo, porotokoro ya lac ikoreshwa kenshi mugutwara imvugo ya T7 RNA polymerase, nayo, ikandukura ingirabuzimafatizo zigenzurwa na T7 zikurikirana.IPTG ikoreshwa mugukurura imvugo ya T7 RNA polymerase, biganisha kumikorere ya gene imvugo.
| Ibigize | C9H18O5S |
| Suzuma | 99% |
| Kugaragara | Ifu yera |
| URUBANZA No. | 367-93-1 |
| Gupakira | Ntoya kandi nini |
| Ubuzima bwa Shelf | Imyaka 2 |
| Ububiko | Ubike ahantu hakonje kandi humye |
| Icyemezo | ISO. |
