2-NITROPHENYL-BETA-D-GLUCOPYRANOSIDE CAS:2816-24-2

2-Nitrophenyl-beta-D-glucopyranoside ni igikoresho cy’ibinyabutabire kigizwe na molekile ya glucopyranoside ifatanye n’itsinda rya nitrophenyl. Ikunze gukoreshwa nk’igice cy’ingenzi mu isuzuma rya enzymatic kugira ngo hamenyekane kandi hamenyekane imikorere ya enzymes nka beta-glucosidase. Itsinda rya nitrophenyl rishobora gucibwamo na enzyme, bigatuma habaho irekurwa ry’ikintu gifite ibara ry’umuhondo gishobora gupimwa hifashishijwe spectrophotometric. Iki gice cy’ibinyabutabire ni ingirakamaro cyane mu kwiga kinetics ya enzymes no gusuzuma neza uburyo enzymes zirinda cyangwa zikora. Ikoreshwa kandi mu bushakashatsi bwa biochemical mu iperereza ku mikorere ya carbohydrates no nk’igice cy’ingenzi cya glycosidic-link.

Gutumiza cyangwa Gusaba Igiciro

Ibisobanuro birambuye ku gicuruzwa

Ibirango by'ibicuruzwa

Ishyirwa mu bikorwa n'ingaruka

Enzyme substrate: ONPG ikunze gukoreshwa nk'inzyme ya beta-galactosidase, enzyme ikoresha hydrolyze ya ONPG kugira ngo ikore ikintu cy'umuhondo (o-nitrophenol) gishobora kugaragara byoroshye hifashishijwe spectrophotometric. Iyi enzyme reaction ishobora gukoreshwa mu gupima imikorere ya beta-galactosidase, bigatuma ONPG iba igikoresho cy'agaciro mu bushakashatsi ku nzyme.

Isuzumabumenyi rya biology ya molekile: ONPG ikoreshwa nk'ishingiro mu isuzumabumenyi rya biology ya molekile zitandukanye, cyane cyane mu isuzumabumenyi rya gene ya beta-galactosidase. Muri izi suzumabumenyi, ishingiro rya ONPG rikoreshwa mu gupima imikorere ya gene ya reporter, ubusanzwe igenzurwa n'urukurikirane runaka rw'inyungu za promoter. Igikorwa cya beta-galactosidase, kigaragazwa n'impinduka z'ibara ziterwa na hydrolysis ya ONPG, gitanga amakuru y'ingirakamaro ku gikorwa cya promoter.

Isesengura ry’igaragara ry’uturemangingo: ONPG ikoreshwa kandi mu isesengura ry’igaragara ry’uturemangingo. Mu guhuza urukurikirane rw’igaragara ry’uturemangingo n’uturemangingo twa beta-galactosidase, abashakashatsi bashobora gupima ibikorwa bya beta-galactosidase bakoresheje ONPG nk’igice cy’ingenzi. Urwego rw’ikorwa rya beta-galactosidase rugaragaza imbaraga n’imikorere y’uturemangingo, bigatuma habaho isuzuma ry’ingano y’igaragara ry’uturemangingo.

Uburyo bwo gusuzuma: ONPG ishobora gukoreshwa mu gusuzuma, cyane cyane mu gutahura udukoko dutera indwara. Bagiteri zitandukanye, nka Escherichia coli n'ubwoko bumwe na bumwe bwa Shigella na Salmonella, zikora beta-galactosidase ishobora gutandukanya ONPG. Iyi hydrolysis reaction ituma habaho impinduka igaragara y'ibara, ishobora gukoreshwa mu kumenya niba izi bagiteri ziri mu bipimo bya muganga.

Urugero rw'ibicuruzwa

Gupakira ibicuruzwa:

Amakuru y'inyongera:

Imiterere	C12H15NO8
Isuzuma	99%
Isura	Ifu y'umuhondo woroshye
Nimero ya CAS	2816-24-2
Gupakira	Bito kandi byinshi
Igihe cyo kuruhuka	Imyaka 2
Ububiko	Bika ahantu hakonje kandi humutse
Icyemezo	ISO.